Stemphylium solani, S. lycopersici and S. botryosum are the causal agents of tomato grey leaf spot, a disease with high incidence and severity within tomato production areas that has a relevant economic impact. Necrotrophic fungi of the genus Stemphylium synthesize secondary metabolites including host (HSTs) and non-host specific toxins (non-HSTs). The purpose is to study the metabolites synthesized and secreted by S. lycopersici isolate CIDEFI- 216 when it is cultured under different conditions and their effect on plant tissue. CIDEFI-216 was grown on media such as V8, potato dextrose agar (PDA) and potato dextrose broth alone or amended with a filtered macerate of a susceptible tomato hybrid leaves (PDB and PDBs).