Response to long-term NaHCO3-derived alkalinity in model Lotus japonicus ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physiological characterization

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cic.isFulltexttrueen
cic.isPeerReviewedtrueen
cic.lugarDesarrolloInstituto de Investigaciones Biotecnológicas - Instituto Tecnológico Chascomús es
cic.versioninfo:eu-repo/semantics/publishedVersionen
dc.date.accessioned2018-05-17T13:38:05Z
dc.date.available2018-05-17T13:38:05Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/7568
dc.titleResponse to long-term NaHCO3-derived alkalinity in model Lotus japonicus ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physiological characterizationen
dc.typeArtículoes
dcterms.abstractThe current knowledge regarding transcriptomic changes induced by alkalinity on plants is scarce and limited to studies where plants were subjected to the alkaline salt for periods not longer than 48 h, so there is no information available regarding the regulation of genes involved in the generation of a new homeostatic cellular condition after long-term alkaline stress. Lotus japonicus is a model legume broadly used to study many important physiological processes including biotic interactions and biotic and abiotic stresses. In the present study, we characterized phenotipically the response to alkaline stress of the most widely used L. japonicus ecotypes, Gifu B-129 and MG-20, and analyzed global transcriptome of plants subjected to 10 mM NaHCO3 during 21 days, by using the Affymetrix Lotus japonicus GeneChipH. Plant growth assessment, gas exchange parameters, chlorophyll a fluorescence transient (OJIP) analysis and metal accumulation supported the notion that MG-20 plants displayed a higher tolerance level to alkaline stress than Gifu B-129. Overall, 407 and 459 probe sets were regulated in MG-20 and Gifu B-129, respectively. The number of probe sets differentially expressed in roots was higher than that of shoots, regardless the ecotype. Gifu B-129 and MG-20 also differed in their regulation of genes that could play important roles in the generation of a new Fe/Zn homeostatic cellular condition, synthesis of plant compounds involved in stress response, protein-degradation, damage repair and root senescence, as well as in glycolysis, gluconeogenesis and TCA. In addition, there were differences between both ecotypes in the expression patterns of putative transcription factors that could determine distinct arrangements of flavonoid and isoflavonoid compounds. Our results provided a set of selected, differentially expressed genes deserving further investigation and suggested that the L. japonicus ecotypes could constitute a useful model to search for common and distinct tolerance mechanisms to long-term alkaline stress response in plants.en
dcterms.creator.authorCampestre, María Paulaes
dcterms.creator.authorBabuin, Maria Florenciaes
dcterms.creator.authorRocco, Rubénes
dcterms.creator.authorBordenave, Cesar Danieles
dcterms.creator.authorEscaray, Francisco Josées
dcterms.creator.authorAntonelli, Cristian Javieres
dcterms.creator.authorCalzadilla, Pabloes
dcterms.creator.authorGárriz, Andréses
dcterms.creator.authorSerna, Evaes
dcterms.creator.authorCarrasco, Pedroes
dcterms.creator.authorRuiz, Oscar Adolfoes
dcterms.creator.authorMenéndez, Ana B.es
dcterms.extent14 p.es
dcterms.identifier.otherdoi:10.1371/journal.pone.0097106es
dcterms.identifier.urlRecurso onlinees
dcterms.isPartOf.issuevol. 9, no. 5es
dcterms.isPartOf.seriesPLoS ONEes
dcterms.issued2014-05
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0)*
dcterms.subjectmicroarrayen
dcterms.subjectalkalinityen
dcterms.subjectLotusen
dcterms.subject.materiaBiotecnología Agropecuariaes

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