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dc.date.accessioned 2021-10-07T18:54:02Z
dc.date.available 2021-10-07T18:54:02Z
dc.identifier.uri https://digital.cic.gba.gob.ar/handle/11746/11328
dc.title Nanostructured fumarate copolymer-chitosan crosslinked scaffold: an in vitro osteochondrogenesis regeneration study en
dc.type Artículo es
dcterms.abstract In the tissue engineering field, the design of the scaffold inspired on the natural occurring tissue is of vital importance. Ideally, the scaffold surface must promote cell growth and differentiation, while promote angiogenesis in the in vivo implant of the scaffold. On the other hand, the material selection must be biocompatible and the degradation times should meet tissue reparation times. In the present work, we developed a nanofibrous scaffold based on chitosan crosslinked with diisopropylfumarate-vinyl acetate copolymer using anodized aluminum oxide (AAO) templates. We have previously demonstrated its biocompatibility properties with low cytotoxicity and proper degradation times. Now, we extended our studies to demonstrate that it can be successfully nanostructured using the AAO templates methodology, obtaining a nanorod-like scaffold with a diameter comparable to those of collagen fibers of the bone matrix (170 and 300 nm). The nanorods obtained presented a very homogeneous pattern in diameter and length, and supports cell attachment and growth. We also found that both osteoblastic and chondroblastic matrix production were promoted on bone marrow progenitor cells and primary condrocytes growing on the scaffolds, respectively. In addition, the nanostructured scaffold presented no cytotoxicity as it was evaluated using a model of macrophages on culture. en
dcterms.extent 570-579 es
dcterms.issued 2018
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-ShareAlike 4.0 International (BY-NC-SA 4.0) es
dcterms.subject Nanostructured Biomaterials en
dcterms.subject Bone regeneration en
dcterms.subject Cartilage regeneration en
dcterms.subject Polyfumarate en
dcterms.subject Chitosan en
cic.version info:eu-repo/semantics/acceptedVersion es
dcterms.creator.author Lastra, María Laura es
dcterms.creator.author Molinuevo, María Silvina es
dcterms.creator.author Blaszczyk Lezak, Iwona es
dcterms.creator.author Mijangos, Carmen es
dcterms.creator.author Cortizo, Ana María es
cic.lugarDesarrollo Laboratorio de Investigación en Osteopatías y Metabolismo Mineral es
dcterms.subject.materia Ciencias Biológicas es
dcterms.identifier.other doi:10.1002/jbm.a.36260 es
dcterms.identifier.other ISSN:1549-3296 es
dcterms.isPartOf.issue vol. 106, no. 2 es
dcterms.isPartOf.series Journal of Biomedical Materials Research Part A es
cic.isPeerReviewed true es
cic.isFulltext true es
cic.institucionOrigen Laboratorio de Investigación en Osteopatías y Metabolismo Mineral es


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