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dc.date.accessioned 2016-08-18T16:31:11Z
dc.date.available 2016-08-18T16:31:11Z
dc.identifier.uri https://digital.cic.gba.gob.ar/handle/11746/3803
dc.title A simple method to assess the oxidative susceptibility of low density lipoproteins en
dc.type Artículo es
dcterms.abstract Background: Oxidative modification of low density lipoproteins (LDL) is recognized as one of the major processes involved in atherogenesis. The in vitro standardized measurement of LDL oxidative susceptibility could thus be of clinical significance. The aim of the present study was to establish a method which would allow the evaluation of oxidative susceptibility of LDL in the general clinical laboratory. Results: LDL was isolated from human plasma by selective precipitation with amphipathic polymers. The ability of LDL to form peroxides was assessed by measuring thiobarbituric acid reactive substances (TBARS) after incubation with Cu2+ and H2O2. Reaction kinetics showed a three-phase pattern (latency, propagation and decomposition phases) which allowed us to select 150 min as the time point to stop the incubation by cooling and EDTA addition. The mixture Cu 2+/H2O2 yielded more lipoperoxides than each one on its own at the same time end-point. Induced peroxidation was measured in normal subjects and in type 2 diabetic patients. In the control group, results were 21.7 ± 1.5 nmol MDA/mg LDL protein, while in the diabetic group results were significantly increased (39.0 ± 3.0 nmol MDA/mg LDL protein; p < 0.001). Conclusion: a simple and useful method is presented for the routine determination of LDL susceptibility to peroxidation in a clinical laboratory. en
dcterms.extent 7 p. es
dcterms.issued 2001-06-20
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.subject hydrogen peroxide en
dcterms.subject low density lipoprotein en
dcterms.subject thiobarbituric acid reactive substance en
dcterms.subject chemical reaction kinetics en
dcterms.subject genetic susceptibility en
dcterms.subject oxidative stress en
cic.version info:eu-repo/semantics/submittedVersion es
dcterms.creator.author Scoccia, Adriana E. es
dcterms.creator.author Molinuevo, María Silvina es
dcterms.creator.author McCarthy, Antonio Desmond es
dcterms.creator.author Cortizo, Ana María es
cic.lugarDesarrollo Universidad Nacional de La Plata es
dcterms.subject.materia Bioquímica y Biología Molecular es
dcterms.subject.materia Ciencias Naturales y Exactas es
dcterms.identifier.url Registro completo es
dcterms.identifier.other 1472-6890 es
dcterms.isPartOf.issue vol. 1 es
dcterms.isPartOf.series BMC Clinical Pathology es
cic.isPeerReviewed true es
cic.isFulltext true es


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