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dc.title Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach en
dc.type Artículo es
dcterms.abstract α-Hemolysin (HlyA) from Escherichia coli is considered as the prototype of a family of toxins called RTX (repeat in toxin), a group of proteins that share genetic and structural features. HlyA is an important virulence factor in E. coli extraintestinal infections, such as meningitis, septicemia and urinary infections. High concentrations of the toxin cause the lysis of several cells such as erythrocytes, granulocytes, monocytes, endothelial and renal epithelial cells of different species. At low concentrations it induces the production of cytokines and apoptosis. Since many of the subcytolytic effects in other cells have been reported to be triggered by the increase of intracellular calcium, we followed the calcium concentration inside the erythrocytes while incubating with sublytic concentrations of HlyA. Calcium concentration was monitored using the calcium indicator Green 1, 2-photon excitation, and fluorescence lifetime imaging microscopy (FLIM). Data were analyzed using the phasor representation. In this report, we present evidence that, at sublytic concentrations, HlyA induces an increase of calcium concentration in rabbit erythrocytes in the first 10 s. Results are discussed in relation to the difficulties of measuring calcium concentrations in erythrocytes where hemoglobin is present, the contribution of the background and the heterogeneity of the response observed in individual cells. © 2011 Sanchez et al. en
dcterms.extent 9 p. es
dcterms.issued 2011
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.publisher Plos One es
dcterms.subject Microscopía Fluorescente es
dcterms.subject Calcio es
dcterms.subject Citotoxinas es
dcterms.subject Microscopía Fluorescente es
cic.version info:eu-repo/semantics/publishedVersion es Sánchez, Susana es Bakás, Laura Susana es Gratton, Enrico es Herlax, Vanesa es
cic.lugarDesarrollo Universidad Nacional de La Plata es
dcterms.subject.materia Bioquímica y Biología Molecular es
dcterms.identifier.url Documento completo es
dcterms.identifier.other ISSN 1932-6203 es
dcterms.identifier.other DOI: 10.1371/journal.pone.0021127 es
dcterms.isPartOf.issue vol. 6, nº 6 es
dcterms.isPartOf.series PLoS ONE es
cic.isPeerReviewed true es
cic.isFulltext true es


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