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Effect of Surface Topography of Collagen Scaffolds on Cytotoxicity and Osteoblast Differentiation

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Resumen

We have developed a biomimetic scaffold for tissue engineering using bovine collagen with different topographic characteristics, using matrices with random or parallel-arranged collagen fibres. Scaffolds were characterized by SEM, water contact angle and mechanical test. Biocompatiblitiy studies were performed with MC3T3E1 pre-osteoblasts, and adhesion, proliferation, differentiation and mineralization were assessed. Random film showed a smooth surface, while the ordered membrane revealed a grooved structure with channels separated 265 m from each other. Water contact angle was dependent on the direction of observation and the mechanical evaluation showed a lower resistance to traction and a greater ductility for the ordered membrane. Adhesion, proliferation, alkaline phosphatase activity and mineralization were significantly improved when cells were grown on the ordered-collagen matrix. There was no significant increase in pro-inflammatory cytokine release from cells grown on both random and ordered-collagen films. This material could be useful for bone tissue regeneration.

Palabras clave
Collagen
Bone Tissue Engineering
Adhesion
Proliferation
Alkaline Phosphatase
Mineralization
Cytotoxocity
http://creativecommons.org/licenses/by/4.0/

Esta obra se publica con la licencia Creative Commons Attribution 4.0 International (BY 4.0)

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