Effect of Surface Topography of Collagen Scaffolds on Cytotoxicity and Osteoblast Differentiation
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2016-10-19T13:17:08Z | |
| dc.date.available | 2016-10-19T13:17:08Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/4454 | |
| dc.title | Effect of Surface Topography of Collagen Scaffolds on Cytotoxicity and Osteoblast Differentiation | en |
| dc.type | Artículo | es |
| dcterms.abstract | We have developed a biomimetic scaffold for tissue engineering using bovine collagen with different topographic characteristics, using matrices with random or parallel-arranged collagen fibres. Scaffolds were characterized by SEM, water contact angle and mechanical test. Biocompatiblitiy studies were performed with MC3T3E1 pre-osteoblasts, and adhesion, proliferation, differentiation and mineralization were assessed. Random film showed a smooth surface, while the ordered membrane revealed a grooved structure with channels separated 265 m from each other. Water contact angle was dependent on the direction of observation and the mechanical evaluation showed a lower resistance to traction and a greater ductility for the ordered membrane. Adhesion, proliferation, alkaline phosphatase activity and mineralization were significantly improved when cells were grown on the ordered-collagen matrix. There was no significant increase in pro-inflammatory cytokine release from cells grown on both random and ordered-collagen films. This material could be useful for bone tissue regeneration. | en |
| dcterms.creator.author | Cortizo, Ana María | es |
| dcterms.creator.author | Ruderman, Graciela | es |
| dcterms.creator.author | Correa, Gimena | es |
| dcterms.creator.author | Mogilner, Inés G. | es |
| dcterms.creator.author | Tolosa, Eduardo J. | es |
| dcterms.extent | 8 p. | es |
| dcterms.isPartOf.issue | vol. 2 | es |
| dcterms.isPartOf.series | Journal of Biomaterials and Tissue Engineering | es |
| dcterms.issued | 2012 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.subject | Collagen | en |
| dcterms.subject | Bone Tissue Engineering | en |
| dcterms.subject | Adhesion | en |
| dcterms.subject | Proliferation | en |
| dcterms.subject | Alkaline Phosphatase | en |
| dcterms.subject | Mineralization | en |
| dcterms.subject | Cytotoxocity | en |
| dcterms.subject.materia | Ciencias Químicas | es |