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dc.title Reduction of l‑phenylalanine in protein hydrolysates using l‑phenylalanine ammonia‑lyase from Rhodosporidium toruloides en
dc.type Artículo es
dcterms.abstract l-Phenylalanine ammonia-lyase (PAL, EC from Rhodosporidium toruloides was utilized to remove l-phenylalanine (l-Phe) from different commercial protein hydrolysates. A casein acid hydrolysate (CAH, l-Phe ~2.28 %) was employed as a model substrate. t-Cinnamic acid resulting from deamination of l-Phe was extracted, analyzed at λ = 290 nm, and used for PAL activity determination. Optimum reaction conditions, optimized using successive Doehlert design, were 35 mg mL-1 of CAH and 800 mU mL-1 of PAL, while temperature and pH were 42 °C and 8.7, respectively. Reaction kinetics of PAL with CAH was determined under optimized conditions. Then, removal of l-Phe from CAH was tested. Results showed that more than 92 % of initial l-Phe was eliminated. Similar results were obtained with other protein hydrolysates. These findings demonstrate that PAL is a useful biocatalyst for l-Phe removal from protein hydrolysates, which can be evaluated as potential ingredients in foodstuffs for PKU patients. es
dcterms.extent 9 p. es
dcterms.issued 2015-08-05
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) es
dcterms.subject Phenylketonuria en
dcterms.subject Phenylalanine ammonialyase en
dcterms.subject Rhodosporidium toruloides en
dcterms.subject Casein acid hydrolysate en
dcterms.subject l-Phe removal en
cic.version info:eu-repo/semantics/publishedVersion es Castañeda, María Teresita es Adachi, Osao es Hours, Roque A. es
cic.lugarDesarrollo Centro de Investigación y Desarrollo en Fermentaciones Industriales es
cic.lugarDesarrollo Universidad Nacional de La Plata es
dcterms.subject.materia Ciencias Químicas es
dcterms.identifier.url Recurso Completo es
dcterms.identifier.other DOI:10.1007/s10295-015-1664-z es
dcterms.isPartOf.issue vol. 42, no.10 es
dcterms.isPartOf.series Journal of Industrial Microbiology and Biotechnology es


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