β-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis

cic.isFulltexttrueen
cic.isPeerReviewedtrueen
cic.lugarDesarrolloCentro de Investigación de Proteínas Vegetales es
cic.versioninfo:eu-repo/semantics/publishedVersionen
dc.date.accessioned2018-05-17T13:11:15Z
dc.date.available2018-05-17T13:11:15Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/7528
dc.titleβ-carbonic anhydrases play a role in salicylic acid perception in Arabidopsisen
dc.typeArtículoes
dcterms.abstractThe plant hormone salicylic acid (SA) is required for defense responses. NON EXPRESSER OF PATHOGENESIS RELATED 1 (NPR1) and NON RECOGNITION OF BTH-4 (NRB4) are required for the response to SA in Arabidopsis (Arabidopsis thaliana). Here, we isolated several interactors of NRB4 using yeast two-hybrid assays. Two of these interactors, βCA1 and βCA2, are β-carbonic anhydrase family proteins. Since double mutant βca1 βca2 plants did not show any obvious phenotype, we investigated other βCAs and found that NRB4 also interacts with βCA3 and βCA4. Moreover, several βCAs interacted with NPR1 in yeast, including one that interacted in a SA-dependent manner. This interaction was abolished in loss-of-function alleles of NPR1. Interactions between βCAs and both NRB4 and NPR1 were also detected in planta, with evidence for a triple interaction, NRB4- βCA1-NPR1. The quintuple mutant βca1 βca2 βca3 βca4 βca6 showed partial insensitivity to SA. These findings suggest that one of the functions of carbonic anhydrases is to modulate the perception of SA in plants.en
dcterms.creator.authorMedina-Puche, Lauraes
dcterms.creator.authorCastello, María Josées
dcterms.creator.authorCanet, Juan Vicentees
dcterms.creator.authorLamilla, Juliánes
dcterms.creator.authorColombo, María Lauraes
dcterms.creator.authorTornero, Pabloes
dcterms.extent29 p.es
dcterms.identifier.otherdoi.10.1371/journal.pone.0181820en
dcterms.identifier.urlRecurso onlinees
dcterms.isPartOf.issuevol. 12, no. 7es
dcterms.isPartOf.seriesPLoS ONEes
dcterms.issued2017-07-28
dcterms.languageIngléses
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectarabidopsis thalianaen
dcterms.subjectyeasten
dcterms.subjectpoint mutationen
dcterms.subjectprotein interactionen
dcterms.subjectphenotypesen
dcterms.subject.materiaAgronomía, reproducción y protección de plantases
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