Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2016-10-18T13:59:20Z | |
| dc.date.available | 2016-10-18T13:59:20Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/4448 | |
| dc.title | Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation | en |
| dc.type | Artículo | es |
| dcterms.abstract | Nitric oxide NO. has been shown to act as a mediator of cytokines in bone tissue. We have previously demonstrated that vanadium compounds are insulin- and growth factor-mimetic compounds in osteoblasts in culture, although high doses are toxic to these cells. In this study, we measured NO production in two osteoblast-like cells UMR106 and MC3T3E1. incubated with different concentrations 2.5–100 mM. of vanadate. Vanadate induced NO release in a biphasic manner, with levels being significantly increased at concentrations over 50 mM. The NO donor, sodium nitroprusside, mimicked the vanadate effect: it inhibited cell growth and alkaline phosphatase activity in a dose-dependent manner. Vanadate enhanced the NO synthases, the endothelial and inducible eNOS and iNOS. isoforms, in a dose-dependent manner. Experiments performed with the ionophore A23187 and EGTA suggested that vanadate-induced NO production involves Ca2q-dependent and -independent mechanisms. Altogether, our results suggest that NO may play a critical role in the bioactivity of vanadium in osteoblast-like cells. q2000 Elsevier Science B.V. All rights reserved. | en |
| dcterms.creator.author | Cortizo, Ana María | es |
| dcterms.creator.author | Caporossi, Mariana | es |
| dcterms.creator.author | Lettieri, Gabriela | es |
| dcterms.creator.author | Etcheverry, Susana B. | es |
| dcterms.extent | 7 p. | es |
| dcterms.isPartOf.issue | vol. 400, no. 1-2 | es |
| dcterms.isPartOf.series | European Journal of Pharmacology | es |
| dcterms.issued | 2000 | |
| dcterms.language | Español | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.subject | Nitric oxide (NO) | en |
| dcterms.subject | Nitric oxide (NO) synthases | en |
| dcterms.subject | Vanadium | en |
| dcterms.subject | Growth | en |
| dcterms.subject | Osteoblast differentiation | en |
| dcterms.subject | Bone | en |
| dcterms.subject | Cytotoxicity | en |
| dcterms.subject.materia | Ciencias Químicas | es |
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