Vanadate-induced nitric oxide production: role in osteoblast growth and differentiation

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloUniversidad Nacional de La Plata es
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dc.date.accessioned2016-10-18T13:59:20Z
dc.date.available2016-10-18T13:59:20Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/4448
dc.titleVanadate-induced nitric oxide production: role in osteoblast growth and differentiationen
dc.typeArtículoes
dcterms.abstractNitric oxide NO. has been shown to act as a mediator of cytokines in bone tissue. We have previously demonstrated that vanadium compounds are insulin- and growth factor-mimetic compounds in osteoblasts in culture, although high doses are toxic to these cells. In this study, we measured NO production in two osteoblast-like cells UMR106 and MC3T3E1. incubated with different concentrations 2.5–100 mM. of vanadate. Vanadate induced NO release in a biphasic manner, with levels being significantly increased at concentrations over 50 mM. The NO donor, sodium nitroprusside, mimicked the vanadate effect: it inhibited cell growth and alkaline phosphatase activity in a dose-dependent manner. Vanadate enhanced the NO synthases, the endothelial and inducible eNOS and iNOS. isoforms, in a dose-dependent manner. Experiments performed with the ionophore A23187 and EGTA suggested that vanadate-induced NO production involves Ca2q-dependent and -independent mechanisms. Altogether, our results suggest that NO may play a critical role in the bioactivity of vanadium in osteoblast-like cells. q2000 Elsevier Science B.V. All rights reserved.en
dcterms.creator.authorCortizo, Ana Maríaes
dcterms.creator.authorCaporossi, Marianaes
dcterms.creator.authorLettieri, Gabrielaes
dcterms.creator.authorEtcheverry, Susana B.es
dcterms.extent7 p.es
dcterms.isPartOf.issuevol. 400, no. 1-2es
dcterms.isPartOf.seriesEuropean Journal of Pharmacologyes
dcterms.issued2000
dcterms.languageEspañoles
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectNitric oxide (NO)en
dcterms.subjectNitric oxide (NO) synthasesen
dcterms.subjectVanadiumen
dcterms.subjectGrowthen
dcterms.subjectOsteoblast differentiationen
dcterms.subjectBoneen
dcterms.subjectCytotoxicityen
dcterms.subject.materiaCiencias Químicases
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