Acceso Abierto

Effects of advanced glycation end-products on the proliferation and differentiation of osteoblast-like cells

Enlace externo

Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unchanged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was found to be depressed after several days incubation. Cellular alkaline phosphatase activity followed a similar pattern: an initial increase induced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decrease in this parameter. 45Ca2+ uptake was only significantly inhibited by advanced glycation end-products after 24 h incubation. These results suggest that advanced glycation end-products directly regulate osteoblast proliferation and differentiation in a dose and time dependent manner.

Palabras clave
advanced glycation endproducts
diabetes mellitus
osteoblastic cells
cell proliferation
cell differentiation

Esta obra se publica con la licencia Creative Commons Attribution 4.0 International (BY 4.0)
Imagen en miniatura