Feasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honey

cic.institucionOrigenCentro de Investigaciones de Fitopatología (CIDEFI)es
cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloCentro de Investigaciones de Fitopatología (CIDEFI)es
cic.versioninfo:eu-repo/semantics/acceptedVersiones
dc.date.accessioned2019-09-04T11:51:59Z
dc.date.available2019-09-04T11:51:59Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/10239
dc.titleFeasibility of using RFLP of PCR-amplified 16S rRNAs for rapid differentiation of aerobic spore-forming bacteria from honeyen
dc.typeArtículoes
dcterms.abstractThis study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources.en
dcterms.creator.authorLópez, Ana Claudiaes
dcterms.creator.authorAlippi, Adriana Mónicaes
dcterms.identifier.otherdoi:10.1016/j.mimet.2019.105690es
dcterms.identifier.otherISSN:0167-7012es
dcterms.identifier.urlRecurso onlinees
dcterms.isPartOf.issue2019es
dcterms.isPartOf.seriesJournal of Microbiological Methodses
dcterms.issued2019-08
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacionales
dcterms.publisherElsevier BVes
dcterms.subjectPCR-RFLPen
dcterms.subjectBacillusen
dcterms.subjectPaenibacillusen
dcterms.subjectBrevibacillusen
dcterms.subjectLysinibacillusen
dcterms.subject.materiaAgronomía, reproducción y protección de plantases
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